BotKitDVT > Plantes de La Réunion > ARBRES ARBUSTES > Erythrina variegata- Nourouc ou Pignon d'Inde / de l'Inde- Fabacée- exo - 2 Sep 2018 at 19:44:18
Topic: Erythrina variegata- Nourouc ou Pignon d'Inde / de l'Inde- Fabacée- exo
Erythrina variegata - Nourouc ou Pignon d'Inde de l'Inde- Fabacée- exo
Grand arbre à tronc et branches épineux. Défeuillé en saison sèche. Grandes feuilles trifoliolées vertes ou parfois panachées de jaune. Grappes de grandes fleurs rouges. Gousses noires, étranglées entre les graines.
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Grand arbre à tronc et branches épineux. Défeuillé en saison sèche. Grandes feuilles trifoliolées vertes ou parfois panachées de jaune. Grappes de grandes fleurs rouges. Gousses noires, étranglées entre les graines.
Pharmacognosy Reviews
Wolters Kluwer -- Medknow Publications
Erythrina variegata Linn: A review on morphology, phytochemistry, and pharmacological aspects
A. Kumar, S. Lingadurai, [...], and N. R. Barman
Additional article information
Abstract
This review gives an account of the current knowledge on the morphology, phytochemistry, and pharmacological aspects of Erythrina variegata. E. variegata also called Erythrina indica is a thorny deciduous tree growing to 60 feet tall. A wide range of chemical compounds have been isolated, mainly alkaloids, flavonoids, triterpenoids, and lectin. Different parts of the plant have been used in traditional medicine as nervine sedative, collyrium in opthalmia, antiasthmatic, antiepileptic, antiseptic, and as an astringent. The alkaloids extracted from the leaves of E. variegata are reported to have anti-inflammatory and analgesic activity. Isoflavonoids isolated from E. variegata having antibacterial and anthelmintic activity. E. variegata shows several other characteristic pharmacological effects like neuromuscular blocking, smooth muscle relaxant, CNS depressant, and hydrocholeretic, which are consistent with the reported uses of the plant extracts in the indigenous system of medicine. Hence the present article includes the detailed exploration of morphology, phytochemistry, and pharmacological aspects of E. variegata in an attempt to provide a direction for further research.
Keywords: Alkaloids, antibacterial, cytotoxicity, Erythrina variegata, isoflavonoids
INTRODUCTION
The genus Erythrina comprises of about 110 species of trees and shrubs. The name “coral tree” is used as a collective term for these plants. Coral tree is indigenous to the Old World tropics, possibly originally from India to Malaysia, but is native of ancient westward to Zanzibar and eastward to eastern Polynesia (the Marquesas). It is typically found on sandy soil in littoral forest, and sometimes in coastal forest up to 250m (800ft) in elevation. The coral tree is cultivated particularly as an ornamental tree and as a shade and soil improvement tree (it fixes nitrogen) for other tree crops such as coffee and cacao. The most attractive type, var. variegata, is grown for its variegated leaves, as well as its seasonal showy red flowers.[1–3] This fast-growing, 50-60 feet tall and wide deciduous tree with green and yellow-variegated, 6-inch-long leaves creates a broad canopy but has spiny branches. In spring, before the leaves appear, coral tree is decorated with showy red blossoms, each flower 2.5 inches long and arranged in dense, six-inch-long racemes. These blooms are followed by 12-inch-long, red/brown seedpods which contain poisonous seeds.[4] Studies on phytochemical of Erythrina variegata species have demonstrated alkaloids and flavonoids as major constituents.[1] Different parts of E. Variegata have used in traditional medicine as nervine sedative, febrifuge, anti-asthmatic and antiepileptic.[5] In the some experiments, it has potential effects for treatment of some diseases like convulsion, fever, inflammation, bacterial infection, insomnia, helminthiasis, cough, cuts and wounds.[6–9]
TAXONOMY
Kingdom: Plantae – Plants
Division: Magnoliophyta – Flowering plants
Class: Magnoliopsida – Dicotyledons
Family: Fabaceae (Legume family)
Subfamily: Papilionoideae
Genus: Erythrina L. – Coral Tree
Species: E. variegata L.
Nonpreferred scientific names
Erythrina corallodendrum var. orientalis L.
Erythrina indica Lam.
Erythrina orientalis (L.) Merrill
Tetradapa javanorum Osbeck
Common names
Coral tree, Indian coral tree, tiger's-claw (English)
Gatae (Samoa, Horne Islands, ‘Uvea, Cook Islands)
Dadap aykam (Java, Indonesia)
MORPHOLOGY
Size
The tree grows up to 60 ft in height, but 33-48ft is more typical, with a spreading crown (except in the cultivar “Tropic Coral”). The dense, oblong to rounded crown is low-branching with many ascending branches.
Flowers
Inflorescence of many-flowered fascicles occurs in terminal or axillary racemes up to 20cm (8 in) or more long. Calyx is top-shaped, deeply split along one side, 1–1.8cm (0.4–0.7 in) long, on a pedicel 2–5mm (0.1–0.2 in) long. Corolla is papilionaceous; standard is short-clawed, ovate to subelliptic, 3–4cm (1.2–1.6 in) long, red-orange with longitudinal white lines; wings are about half as long as the standard, greenish to pale red; keel is as long as the wings, greenish to pale red. Ovary is superior, stamens 10, diadelphous, with 9 fused together at the base, enclosed within the keel. Flowering is reported from July to November in the southern hemisphere and 6 months later in the northern hemisphere.
Leaves
Leaves are trifoliate, alternate; rachis is mostly 10–20 cm (4–8 in) long; blades are ovate to rhomboid, 8–18 cm (3.2–7.2 in) long; lateral ones are smaller than the terminal one, petiolules 6–13 mm long, with vegetative parts finely pubescent. They are deciduous just before and during the flowering season, except for “tropic coral,” which has been reported by some authors to not drop its leaves, while other sources have noted its deciduous habit. E. variegata retains its leaves better than other Erythrina species in Hawai’i. Low temperatures, powdery mildew, and/or drought combined with very windy conditions will accelerate leaf drop and retard the development of new leaves.
Fruit
Fruit a compressed, narrowly oblong pod 10–14 cm (4–5.6 in) long, sterile in the basal portion, and not constricted between the 5–10 dark brown seeds. The fruits are ripe from October to November in the Southern Hemisphere and March to April in the Northern Hemisphere, but they often remain on the tree for several months longer.
Seeds
Seeds are kidney-shaped, dark purple to red, and 1–1.5 cm (0.4–0.6 in) in length. These simply fall to the ground and may be washed away (they have been seawater-dispersed over their native range). There are 1450–5000 seeds/kg (660–2270 seeds/lb).[3,4]
PHYTOCONSTITUENTS
Alkaloids, flavonoids, pterocarpans, triterpenes, steroids, alkyl trans-ferulates, proteins, and lecithin [Figure 1] are founds in the genus. Literature survey has revealed that a number of reports are available on E. variegata.
Figure 1
Figure 1
Few isolated phytconstituents of E. variegata
Alkaloids
The plant is a rich source of alkaloids (2.5%). (+)-3-Demethoxyerythratidinone, (+)-erythraline, (+)-erythramine, (+)-erythrinine, (+)-erythratidinone, (+)-erysonine, (+)-erysotine, (+)-erysodine, (+)-erysovine, (+)-11-hydroxy- epi- erythratidine, (+)-erythratidine, (+)-epi- erythratidine, (+)-erysodienone, (+)-erysotrine, (+)-erysopitine, (+)-11-β-hydroxyerysotrine[(+)-erythrartine] are the tetracyclic alkaloids isolated from the various parts of plants[7] and scoulerine, (+)coreximine, l-reticuline, and erybidine isolated from leaves.[10] The ethanolic extract yielded chloroform-soluble and water-soluble bases, identified as erysovine and stachydrine.[11] Eight spiroamine alkaloids along with three carboxylated indole-3-alkylmamines – hypaphorine, its methyl ester and N, N-dimethyltryptophan isolated from various parts of plant.[8] Presences of isoquinoline (erythritol) and isococcolinine alkaloids are also reported in the studies.[12]
Flavonoids
Flavonoids are chemical phenylbenzopyrones, which, usually conjugated with sugars, are present in all vascular plants.[13] Isoflavonoids are reported to be the major phytoconstituents of E. Variegata. It contains mainly erythrinins A, B, and C, osajin and alpinum isoflavone, in addition to the styrene oxyresveratrol and dihydrostilbene dihydroxyresveratrol. Linear pyranoisoflavones, robustone and 4-O-methylalpinum isoflavone are also isolated from the plant.[14,15] The previous studies that reported erycricstagallin, orientanol B, erystagallin A,[16] stigmasterol, campesterol,[11] stigmoidins A, B, and C, phaseollin, 3-β-acetoxy-B-norcholest-5-ene, docosanoic methyl ester, 29-norcycloartenol, β-sitosterol and its archidate, and capric acid[7] as main components refuted by recent well-documented and reliable investigations. Presence of flavonoid abyssinone V, erycricstagallin and 4-hydroxy-6, 3, 5-triprenylisoflavonone was confirmed in other studies.[17] In recent studies two new diphenylpropan-1,2-diols, eryvarinols A and B, three new isoflavonoids, eryvarins M-O, two new 2-arylbenzofurans, eryvarins P and Q and a 3-aryl-2,3-dihydrobenzofuran, eryvarin R were isolated from the roots of E. variegata and their structures were elucidated on the basis of spectroscopic and chemical evidence.[18,19] Bioassay-directed fractionation of the stem bark extract of E. variegata has resulted in the isolation of three new isoflavones: 5,4’-dihydroxy-8-(3,3-dimethylallyl)-2”ethoxyisopropylfurano[ 4, 5:6, 7 ]isoflavone, 5,7,4’-trihydroxy-6-(3,3- dimethylallyloxiranylmetyl) isoflavone, 5,4’-dihydroxy-8-(3,3 dimethylallyl)-2”-hydroxymethyl-2”methylpyranol [5, 6:6, 7] isoflavone and a new isoflavanone, 5,4’dihydroxy-2”methoxy-8-(3,3-dimethylallyl)-2”,2”-dimethylpyranol [5, 6:6, 7] isoflavanone, together with seven known compounds, euchrenone b10, isoerysenegalensein E, wighteone, laburnetin, lupiwighteone, erythrodiol, and oleanolic acid.[20] Other newly reported isoflavonoids of E. Variegata are epilupeol, 6-hydroxygenistein, and 3β, 28-dihydroxyolean-12-ene.[15]
Miscellaneous phytoconstituents
Various other constituents, which have been reported from E. variegata include erythrabyssin II, dihydrofolinin,[21] octacosyl ferulate, wax alcohol, wax acids, alkyl ferulates, and alkyl phenolates.[11] Seeds content moisture (3.8%), crude protein (31.2%), pentosan (11.9%), and water-soluble gum (1.6%). The amino acid composition of seed protein is as follows: alanine (7.2), arginine (3.4), aspartic acid (12.9), glutamic acid (13.4), glycine (7.6), histidine (3.9), isoleucine (3.6), leucine (7.1), lysine (5.1), methionine (0.5), phenylalanine (3.3), proline (4.7), serine (7.1), threonine (5.7), tyrosine (2.2), and valine (4.8) g/100g. The seeds also contain isolecitins (EVLI, EVLII and EVLIII), the kuntz-type trypsin inhibitors (ETIa and ETIb) chymotrypsin inhibitor (ECI).[22]
PHARMACOLOGICAL ASPECTS
Indian coral tree in the South Pacific, in Pohnpei the leaves are reportedly used to make a drink to cure curses, and the smoke from smoldering leaves, bark, or roots is inhaled for the same purpose. In Yap, the leaves and bark are reportedly used as a potion to treat stomachache. In Tonga the bark is mixed with others and used to treat stomachache. In Samoa, the leaves are occasionally used to treat eye ailments, and the bark is applied to swellings. In India, China, and Southeast Asia, the bark and leaves are used in many traditional medicines, including one said to destroy pathogenic parasites and relieve joint pain; the juice from the leaves is mixed with honey and ingested to treat tapeworm, roundworm, and threadworm in India; women take this juice to stimulate lactation and menstruation; it is commonly mixed with castor oil to treat dysentery; a warm poultice of the leaves is applied externally to relieve rheumatic joints; and the bark is used as a laxative, diuretic, and expectorant.[23,24]
Antibacterial/dental caries prevention
Isoflavonoids isolated from E. variegata has been screened for antibacterial activity against methicillin-resistant Staphylococcus aureus and various other strains. Of the active compounds, erycristagallin and orientanol B showed the highest antibacterial activity. The antibacterial effect of erycristagallin to mutans streptococci was based on a bactericidal action. Erycristagallin (6.25μg/ml: MIC) completely inhibited incorporation of radio-labeled thymidine into Streptococcus mutans cells. Incorporation of radio-labeled glucose into bacterial cells was also strongly inhibited at MIC, and 1/2 MIC of the compound reduced the incorporation approximately by half. The findings indicate that erycristagallin has a potential as potent phytochemical agent for the prevention of dental caries by inhibiting the growth of cariogenic bacteria and by interfering with incorporation of glucose responsible for production of organic acids.[15,16]
Antioxidant
The generation of free radicals and other reactive oxygen species in the body is compensated by an elaborate endogenous antioxidant system. However, due to many environmental, lifestyle, and pathological situations, excess radicals can accumulate, resulting in oxidative stress. The potential value of antioxidants in eradicating oxidative stress has provoked researchers to investigate for natural compounds with potent antioxidative activity but low cytotoxocity. Crude extract obtained from the E. variegata evaluated for their radical scavenging properties and assessed that it could be rich source of natural oxidants for applications.[5]
Analgesic and anti-inflammatory
The alkaloids extracted from the leaves of E. variegata are reported to have anti-inflammatory activity. The leaves and barks are also used in fever and rheumatism.[7] It has been reported that in acetic acid induced writhing model the methanolic extract of the leaf of E. variegata at a dose of 500 mg/kg showed significant antinociceptive activity with 49.03% inhibition of writhing response. The results were statistically significant (P < 0.01) in comparison to the control. In radiant heat tail-flick model, the extract also showed significant increase in the tail flick latency at a dose of 500mg/kg body weight with 36.02% elongation of tail flick time.[9]
Cardiovascular effects
Despite improved pharmacotherapies and mechanical treatments, cardiovascular disease remains a principal cause of morbidity and mortality worldwide, with every chance that this burden will increase.[25] The intravenous administration of the E. variegata seed extract at a dose, varying from 0.1 to 0.4 mg/kg produced a sharp and short-lived fall in BP, both in cats and rats in acute experiments. On the isolated frog hearts, the extract has no action in smaller dose but at a dose of 5 mg resulted a complete but reversible block of the heart.[26]
CNS effects
In the study total alkaloid fraction from the bark showed several characteristic pharmacological effects: neuromuscular blocking, CNS depressant, and anticonvulsant effects which are consistent with the reported uses of the plant extracts in the indigenous system of medicine.[8] E. variegata also causes passivity and decreases spontaneous activity with positive grip strength. This indicates CNS relaxant activity (anxiolytic) of this plant.[5] The current therapeutic treatment of epilepsy with modern antiepileptic drugs (AEDs) is associated with side-effects, dose-related teratogenic effects, and approximately 30% of the patients continue to have seizures with current AEDs therapy. Natural products from folk remedies have contributed significantly in the discovery of modern drugs and can be an alternative source for the discovery of AEDs with novel structures and better safety and efficacy profiles. Evidence for anticonvulsant activity of E. variegata in the clonic seizure of pentylenetetrazole model has been tested in mice. As the protective effects of E. variegata in clonic seizure, it suggests that it could be useful for treatment of absence seizure.[27]
Smooth muscle relaxant
Total alkaloidal fraction from bark caused smooth muscle relaxation of isolated rabbit ileum and inhibited spontaneous rhythmic contraction of isolated rat uterus in concentration of 0.5–2.0mg/mL. E. variegata behaves like a spasmolytic agent due to its relaxing activity; therefore, it can play an important role in conditions like diarrhea or spasm or colic pain.[8]
Calcium homeostasis
E. variegata extracts were evaluated on calcium homeostasis in overiectomized rats and the regulation on gene expression in duodenum and kidney. It improve the serum Ca level and inhibit the urinary Ca excretion in OVX rats, and this might be due to the upregulation of E. variegata on VDR mRNA expression in duodenum and CaBP-9k mRNA expression in kidney.[28]
Antiosteoporotic effect
Study showed that E. variegata could suppress the high rate of bone turnover induced by estrogen deficiency, inhibit bone loss and improve the biomechanical properties of bone in the lab rats.[29]
Trypsin/proteinase inhibitors
Study indicates that erythrina kunitz proteinase inhibitors possess different potency toward serine proteinases in the blood coagulation and fibrinolytic systems, in spite of their high similarity in amino acid sequence.[30]
Cytotoxicity
The lethality of the n-hexane, carbon tetrachloride, chloroform, and aqueous soluble fractions of the methanolic extract to brine shrimp was evaluated on Artemia salina. The LC50 were found to be 36.68, 4.67, 7.733, and 14.289 μg/mL for n-hexane, carbon tetrachloride, chloroform, and aqueous fractions, respectively. In comparison with the positive control (vincristine sulphate), the cytotoxicity exhibited by the carbon tetrachloride and chloroform-soluble fractions of the methanolic extract was significant. This clearly indicated the presence of potent bioactive principles in these extractives, which might be very useful as antiproliferative, antitumor, pesticidal, and other bioactive agents.[15]
Cadmium removal
It has been reported that E. variegata leaf powder can be used as an effective adsorbent for the removal of cadmium, a priority pollutant from its aqueous solutions. The equilibrium agitation time for the cadmium biosorption onto E. variegata leaf powder is 50 min. The percentage removal increases with a decrease in the size of the adsorbent from 212 to 53μm. The percentage removal is increased with an increase in the adsorbent dosage from 10 to 50g/L. Percentage removal is increased significantly with increase in pH from 2 to 4. The percentage removal decreases as pH is increased beyond 7.[31]
Zinc removal
Pollution is the addition of unwanted and undesirable foreign matter to environment as a result of enormous industrial development and modernization. The discharge of untreated solid, liquid, and gaseous wastes that contaminate the physiological and ecological environment is the greatest threat to mankind. Waste water contaminated with heavy metals is one of the most common environmental problems due to their toxicity. Zinc finds its way into water bodies through effluents from smelters, mining, processing plants, paints and pigments, pesticides, and galvanizing units. When zinc is present in the wastewater beyond the permissible limits of concentration, it becomes harmful to the living organisms. Successful metal biosorption has been reported by a variety of biological materials. Evidence for metal biosorption of E. variegata leaf powder has been reported in recent studies. It is observed that there is a significant increase in percentage removal of Zn(II) as pH increases from 2 to 3 and attains maximum when pH is 7. The agitation time is to be 60 min.[32]
CONCLUSION
E. variegata has been ethnomedicinally used as a therapeutic agent for a variety of diseases, as we have illustrated in this article. Moreover, numerous research works have proven its uses beyond the ethnomedicinal ones in experimental animals. Alkaloids and flavonoids which were isolated from this plant may be responsible for its pharmacological activities. The road ahead is to establish specific bioactive molecules, which might be responsible for these actions. Therefore the cultivation, collection, and further pharmacological exploration of E. variegata are essential.
ABBREVIATIONS USED
E. varigata, Erythrina variegata; MIC, minimum inhibitory concentration; AEDs, antiepileptic drugs; OVX, ovariectomized; VDR mRNA, vitamin D receptor mRNA; CaBP-9k, calcium binding protein; LC50, lethal concentration 50.
Footnotes
Source of Support: Nil
Conflict of Interest: None declared
Article information
Pharmacogn Rev. 2010 Jul-Dec; 4(8): 147–152.
doi: 10.4103/0973-7847.70908
PMCID: PMC3249914
A. Kumar, S. Lingadurai, A. Jain, and N. R. Barman
Department of Pharmacology, Himalayan Pharmacy Institute, Majhitar, Rangpo, East Sikkim, India.
Address for correspondence: Dr. Sutharson Lingadurai, E-mail: ni.oc.oohay@nos_kajpa
Received 2010 Mar 30; Revised 2010 Apr 2
Copyright : © Pharmacognosy Reviews
This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This article has been cited by other articles in PMC.
Articles from Pharmacognosy Reviews are provided here courtesy of Wolters Kluwer -- Medknow Publications
REFERENCES
1. Cui L, Thuong PT, Fomum ZT, Oh WK. A new erythrinan alkaloid from the seed of Erythrina addisoniae. Arch Pharm Res. 2009;32:325–8. [PubMed]
2. Rukachaisirikul T, Saekee A, Tharibun C, Watkuolham S, Suksamrarn A. Biological activities of the chemical constituents of Erythrina stricta and Erythrina subumbrans. Arch Pharm Res. 2007;30:1398–403. [PubMed]
3. Agroforestry.net. Holualoa, Hawaii 96725 USA: The Traditional Tree Initiative. c1997-2010. [last updated on 2010 Jan 26, last cited on 2010 Feb 5]. Available from: http://www.traditionaltree.org/
4. Hort.ifas.ufl.edu. University of Florida: Environmental Horticulture. c2008.. [last updated on 2010 Jan 12. last cited on 2010 Jan 27]. Available from: http://hort.ufl.edu/
5. Anwar M. Ph D. Thesis. Karachi, Pakistan: Faculty of Pharmacy, University of Karachi; 2006. The pharmacognostic and pharmacological studies on medicinal valued herbal drugs, Erythrina variegata Var. Orientalis, Matricaria chamommilla, Psoralea corylifolia and Chenopodium album.
6. Warrier PK, Nambiar VP, Ramankutty C, editors. Indian medicinal plants a compendium of 500 species. 1st ed. Hyderabad: Orient Longman Limited; 1994. Anonymous; p. 1994.
7. Gupta VK, editor. The Wealth of India (A dictionary of Indian raw materials and industrial product) 3rd ed. New Delhi: National Institute of Science Communication and Council of Industrial and Scientific Research; 2002. Anonymous.
8. Ghosal S, Dutta SK, Bhathacharya SK. Erythrina - chemical and pharmacological evaluation II: Alkaloids of Erythrina variegata L. J Pharm Sci. 1972;61:1274–7. [PubMed]
9. Haque R, Ali MS, Saha A, Allimuzzaman M. Analgesic activity of methanolic extract of the leaf of Erythrina variegata. J Pharm Sci. 2006;5:77–9.
10. Rastogi RP, Mehrotra BN, editors. Compendium of Indian medicinal plants. 2nd ed. Lucknow: Central Drug Research Institute and New Delhi, National Institute of Science Communication and Info Resources; 2006. Anonymous.
11. Singh H, Chawla AS, Jindal AK, Conner AH, Rowe JW. Investigation of Erythrina spp. VII: Chemical constituents of Erythrina variegata Var. orientalis bark. Lloydia. 1975;38:97–100. [PubMed]
12. Chawla HM, Sharma SK. Erythritol, a new isoquinoline alkaloid from Erythrina variegata flower. Fitoterapia. 1993;64:15–7.
13. Zanoli P, Avallone R, Baraldi M. Behavioral characterisation of the flavonoids apigenin and chrysin. Fitoterapia. 2000;71:S117–23. [PubMed]
14. Chawla AS, Krishnan TR, Jackson AH, Scalabrin DA. Alkaloidal constituents of Erythrina variegata bark. Planta Med. 1988;54:526–8. [PubMed]
15. Rahman MZ, Sultana SZ, Faruquee CF, Ferdous F, Rahman MS, Islam MS, et al. Phytochemical and Biological investigation of Erythrina variegata. Saudi Pharm J. 2007;15:140–5.
16. Sato M, Tanaka H, Fujiwara S, Hirata M, Yamaguchi R, Etho H, et al. Antibacterial property of isoflavonoids isolated from Erythrina variegata against cariogenic oral bacteria. Phytomedicine. 2003;10:427–33. [PubMed]
17. Hegde VR, Dai P, Patel MJ, Puar MS, Das P, Pai J, et al. Phospholipase A2 Inhibitors from an Erythrina Species from Samoa. J Nat Prod. 1997;60:537–9.
18. Tanaka H, Hirata M, Etoh H, Watanabe N, Shimizu H, Ahmad M, et al. Two Diphenylpropan-1,2-diol Syringates from the Roots of Erythrina variegata. J Nat Prod. 2002;65:1933–5. [PubMed]
19. Tanaka H, Hirata M, Etoh H, Sako M, Sato M, Murata J, et al. Six new constituents from the Roots of Erythrina variegata. Chem Biodivers. 2004;1:1101–8. [PubMed]
20. Xiaoli L, Naili W, Sau WM, Chen AS, Xinsheng Y. Four Isoflavonoids from the stem bark of Erythrina variegata. Chem Pharm Bull (Tokyo) 2006;54:570–3. [PubMed]
21. Ahmad WY, Said IM, Soon SY, Takayama H, Kitajima M, Aimi N. A Petrocarpan from Erythrina variegata. Online J Biol Sci. 2002;2:542–4.
22. Samanta TD, Laskar S. Chemical investigation of Erythrina variegata Linn. seed proteins. Food Chem. 2008;114:212–6.
23. Gurung BJ. The medicinal plants of the Sikkim Himalaya. 2nd ed. Kolkata: Subhash Goel Publication; 2002.
24. Ayushveda.com. Ayushveda health and lifestyle portal. c2010. [last cited on 2010 Feb 7]. Available from: http://www.ayushveda.com/
25. Benson VL, Khachigian LM, Lowe HC. DNAzymes and cardiovascular disease. Br J Pharmacol. 2008;154:741–8. [PMC free article] [PubMed]
26. Chatterjee Gk, Gurman TK, Chaudhury AK, Pal SP. Preliminary pharmacological screening of Erythrina variegata Var. Orientalis (Syn. E. indica) seeds. Indian J Pharm. 1981;11:153–8.
27. Bhattacharya SK, DeBnath PK, Sanyal Ak, Ghoshal S. Pharmacological studies of the alkaloids of Erythrina variegata (mandar) J Res Indian Med. 1971;6:235–41.
28. Zhang Y, Li XL, Huang WX. Effect of Erythrina variegata on Ca2+ homeostasis in ovariectomized rats and action mechanism. Zhongguo Zhong Yao Za Zhi. 2007;32:627–30. [PubMed]
29. Zhang Y, Li XL, Lai WP, Chen B, Chow HK, Wu CF, et al. Anti-osteoporotic effect of Erythrina variegata L. in ovariectomized rats. J Ethnopharmacol. 2007;109:165–9. [PubMed]
30. Nakagaki T, Shibuya Y, Kouzuma Y, Yamasaki N, Kimura M. Inhibitory potency of Erythrina variegata proteinase inhibitors toward serine proteinases in the blood coagulation and fibrinolytic systems. Biosci Biotechnol Biochem. 1996;60:1383–5. [PubMed]
31. Kumar PR, Rao MV, Babu NC, Kumar PV, Venkateswarlu P. Utilization of Erythrina variegata orientalis leaf powder for the removal of cadmium. Indian J Chem Technol. 2009;16:308–16.
32. Venkateswarlu1 P, Durga GV, Babu NC, Rao MV. Biosorption of Zn(II) from an aqueous solution by Erythrina variegata orientalis leaf powder. Int J Phys Sci. 2008;3:197–204.
